Molecular analysis of metallo-β-lactamase genes in some gram-negative bacteria and examination of the phylogenetic relationships of isolates

<b>Aim:</b> This study aimed to determine the susceptibility of carbapenem-resistant Gr (-) bacilli isolated from various clinical infections antibiotics and identify genes causing carbapenem resistance their clonal relationships elucidate distribution in community and/or hospital-acquired strains.<br /> <b>Material methods:</b> In this study, antibiotic susceptibilities 450 specimens at Cukurova University, Faculty Medicine, Balcali Hospital, were investigated using phenotypic methods. The presence carbapenems β-lactamase searched polymerase chain reaction (PCR) sequence analysis Pulsed-field gel electrophoresis (PFGE) method was used evaluate phylogenetic relationship isolates.<br <b>Results:</b> Based on results, it determined that 99.23% strains had gained meropenem, whereas 5.38% developed colistin. most dominant all isolates OXA-51, OXA-23-like OXA-24-like.<br <b>Conclusion:</b> It observed only could be safely addition, when examined, found clones considered closely related persisted, these settled different clinics our hospital.